Plasmid_Backbone

Part:BBa_K4294094:Design

Designed by: Nadler et al, David Savage   Group: iGEM22_Athens   (2022-10-12)


pTKEI-Dest


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1911
    Illegal XbaI site found at 1884
    Illegal PstI site found at 1872
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1911
    Illegal PstI site found at 1872
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1911
    Illegal BamHI site found at 1890
    Illegal BamHI site found at 3720
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1911
    Illegal XbaI site found at 1884
    Illegal PstI site found at 1872
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1911
    Illegal XbaI site found at 1884
    Illegal PstI site found at 1872
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 178


Design Notes

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Source

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References